Composite

Part:BBa_K1899005:Design

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-10)


J23101-B0032-TetR-B1006- phlFp -GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1539


Design Notes

The j23101 perhaps is too strong and produce too many tetR, which is toxic and consequently during characterisation, the E. coli (strain: jw0336) grows slower than the normal one. This makes us difficult to compare with the negative control( BBa_E0240, GFP generator) and PphlF-GFP, and observe any interference by tetR on PphlF.


Source

iGEM

References